Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermat
男性生育力保存
摘要 目的：评价一种新型的冷冻载体cell sleeper用于微量人类精子的冷冻效果。方法：将6O例患者的精液标本液化，PureSperm梯度离心处理后进行两部分实验。第一部分运用cell sleeper在不同液量(O．5 1，1 1和21)含有冷冻保护剂的液滴进行精子冷冻，液氮中冷冻2周，比较冻融前后各组精子存活率和活力，以找到cellsleeper用于微量精子冷冻的最佳液量条件。在第一部分实验摸索的液量条件下，进行第二部分cell
Abstract Despite broad utilization of sperm cryopreservation, little progress has been made to modify freezing protocols or to improve rates of sperm survival. Vitrification is an alternative method for freezing human sp
background: Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreser
【摘要】精子冷冻是人类辅助生殖领域中日益成熟的技术，但是常规精子冷冻方案却不适用于稀少精子冷冻，解决稀少精子冷冻技术将在很大程度上改善临床治疗少、弱精子症和无精子症患者的不育问题。寻找合适冷冻载体是目前稀少精子冷冻的研究重点之一，本文就近年来稀少精子不同载体冷冻方法: 空卵膜载体冷冻法、微滴冷冻法、其他微型载体冷冻法和睾丸组织冷冻以及睾丸精子和附睾精子的冷冻效果作一综述。 点击查看PDF全文并下载
Objective: To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility. Design: Retrospective case series. Setting: IVF unit of
Abstract Conventional freezing procedures and containers are not appropriate for spermatozoa from the testis because of their low number and poor in-situ motility, and various types of container have been utilized to fre
【摘要】 目的: 通过研究对无精子症患者实施睾丸活检或其他手术时冷冻睾丸精子经复苏后行卵细胞胞质内单精子注射( ICSI) 助孕的临床效果，探讨冻存睾丸精子作为男性生殖力储备的有效性。 方法: 回顾性分析了在本院实施睾丸活检或其他手术时冷冻睾丸精子的患者96 例，其中的55 例已在本中心复苏冷冻精子行ICSI 助孕共60 个周期，评估其冷冻精子复苏、卵子受精、卵裂、可移植胚胎、优质胚胎、临床妊娠及其分娩情况。 结果: 复苏冻存睾丸精子6
第 2 页 共 2 页