精子与睾丸组织的冷冻与复苏是目前男性生育力保护的主要方法，男性进入青春期后，睾丸生精上皮中精原干细胞即开始精子发生的过程，经过约64天的发育，完成减数分裂并最终形成精子。在我国，一般13 岁以后的青少年男性精液中即可见到精子，此时，如需要进行生育力保存，可采集新鲜精液进行冷冻。而对于青春期前的男孩，如恶性肿瘤患者放化疗之前，则需冷冻睾丸组织来进行生育力保护。 点击查看PDF全文并下载
生育力保存
Abstract The purpose of this study is to develop a novel method for the cryopreservation and efficient post-thaw recovery of individual or small numbers of human spermatozoa. Spermatozoa equilibrated in cryoprotectant bu
Objective: To develop an efficient cryopreservation method using a single sperm. Design: Experimental study. Setting: Laboratory of a private institute. Patient(s): A fertile donor. Intervention(s): We produced hollow-co
Human spermatozoa vitrified in the absence of permeable cryoprotectants: birth of two healthy babies
Abstract. Herein, we report the birth of two healthy babies to a woman following intracytoplasmic sperm injection (ICSI) using motile spermatozoa vitrified without permeable cryoprotectants. Spermatozoa (in a case of oli
Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermat
摘要 目的：评价一种新型的冷冻载体cell sleeper用于微量人类精子的冷冻效果。方法：将6O例患者的精液标本液化，PureSperm梯度离心处理后进行两部分实验。第一部分运用cell sleeper在不同液量(O．5 1，1 1和21)含有冷冻保护剂的液滴进行精子冷冻，液氮中冷冻2周，比较冻融前后各组精子存活率和活力，以找到cellsleeper用于微量精子冷冻的最佳液量条件。在第一部分实验摸索的液量条件下，进行第二部分cell
Abstract Despite broad utilization of sperm cryopreservation, little progress has been made to modify freezing protocols or to improve rates of sperm survival. Vitrification is an alternative method for freezing human sp
background: Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreser
【摘要】精子冷冻是人类辅助生殖领域中日益成熟的技术，但是常规精子冷冻方案却不适用于稀少精子冷冻，解决稀少精子冷冻技术将在很大程度上改善临床治疗少、弱精子症和无精子症患者的不育问题。寻找合适冷冻载体是目前稀少精子冷冻的研究重点之一，本文就近年来稀少精子不同载体冷冻方法: 空卵膜载体冷冻法、微滴冷冻法、其他微型载体冷冻法和睾丸组织冷冻以及睾丸精子和附睾精子的冷冻效果作一综述。 点击查看PDF全文并下载