精子冷冻保存

点击查看: 非梗阻性无精子症患者的福音——大妇儿开展单精子冷冻技术

Abstract The purpose of this study is to develop a novel method for the cryopreservation and efficient post-thaw recovery of individual or small numbers of human spermatozoa. Spermatozoa equilibrated in cryoprotectant bu

Objective: To develop an efficient cryopreservation method using a single sperm. Design: Experimental study. Setting: Laboratory of a private institute. Patient(s): A fertile donor. Intervention(s): We produced hollow-co

Abstract. Herein, we report the birth of two healthy babies to a woman following intracytoplasmic sperm injection (ICSI) using motile spermatozoa vitrified without permeable cryoprotectants. Spermatozoa (in a case of oli

Abstract Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chi

Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermat

摘要 目的：评价一种新型的冷冻载体cell sleeper用于微量人类精子的冷冻效果。方法：将6O例患者的精液标本液化，PureSperm梯度离心处理后进行两部分实验。第一部分运用cell sleeper在不同液量(O．5 1，1 1和21)含有冷冻保护剂的液滴进行精子冷冻，液氮中冷冻2周，比较冻融前后各组精子存活率和活力，以找到cellsleeper用于微量精子冷冻的最佳液量条件。在第一部分实验摸索的液量条件下，进行第二部分cell

Abstract Despite broad utilization of sperm cryopreservation, little progress has been made to modify freezing protocols or to improve rates of sperm survival. Vitrification is an alternative method for freezing human sp

background: Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreser

【摘要】精子冷冻是人类辅助生殖领域中日益成熟的技术，但是常规精子冷冻方案却不适用于稀少精子冷冻，解决稀少精子冷冻技术将在很大程度上改善临床治疗少、弱精子症和无精子症患者的不育问题。寻找合适冷冻载体是目前稀少精子冷冻的研究重点之一，本文就近年来稀少精子不同载体冷冻方法: 空卵膜载体冷冻法、微滴冷冻法、其他微型载体冷冻法和睾丸组织冷冻以及睾丸精子和附睾精子的冷冻效果作一综述。 点击查看PDF全文并下载